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V/2 [1 record]

Record 1 2011-07-05

English

Subject field(s)
  • Biochemistry
  • Biological Sciences
DEF

Velocity of an enzyme reaction when the substrate concentration reaches a concentration equal to the Michaelis-Menten constant (Km).

CONT

The substrate concentration yielding half-maximal velocity provides a useful index for the analysis of some enzyme regulatory mechanisms... Some allosteric enzymes respond to the binding of a modulator with a change in the apparent Km for the substrate, without change in Vmax. A negative modulator will then produce an increase in the apparent Km and a positive modulator a decrease in the apparent Km. Here the term "apparent Km" refers to the substrate concentration giving half-maximal velocity; it cannot be used to calculate enzyme since the vo-vs.-(s) relationship is not a rectangular hyperbola, as required by the Michaelis-Menten assumptions and equations.

OBS

V/2 and 1/2Vmax are abbreviations of half the maximal velocity.

French

Domaine(s)
  • Biochimie
  • Sciences biologiques
DEF

Vitesse d'une réaction enzymatique lorsque la concentration en substrat est égale à la constante de Michaelis (Km).

CONT

L'inhibition compétitive étant réversible, la vitesse maximale correspondant à une quantité élevée de substrat est la même qu'en l'absence d'inhibiteur. Par ailleurs, l'enzyme étant partiellement combinée à l'inhibiteur, la quantité de substrat nécessaire pour obtenir la moitié de la vitesse maximale augmente, donc la Km est plus élevée.

Spanish

Campo(s) temático(s)
  • Bioquímica
  • Ciencias biológicas
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