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CESIUM CHLORIDE CENTRIFUGATION [1 record]
Record 1 - internal organization data 1999-02-03
Record 1, English
Record 1, Subject field(s)
- Genetics
Record 1, Main entry term, English
- cesium chloride centrifugation
1, record 1, English, cesium%20chloride%20centrifugation
correct
Record 1, Abbreviations, English
Record 1, Synonyms, English
- CsCl centrifugation 2, record 1, English, CsCl%20centrifugation
correct
- equilibrium centrifugation in CsCl 2, record 1, English, equilibrium%20centrifugation%20in%20CsCl
correct
Record 1, Textual support, English
Record number: 1, Textual support number: 1 DEF
A common type of equilibrium centrifugation used to separate molecules by their density: they move to the region of the centrifuge cell in which their density equals that of the solution. 2, record 1, English, - cesium%20chloride%20centrifugation
Record 1, French
Record 1, Domaine(s)
- Génétique
Record 1, Main entry term, French
- centrifugation isopycnique en gradient de chlorure de césium
1, record 1, French, centrifugation%20isopycnique%20en%20gradient%20de%20chlorure%20de%20c%C3%A9sium
correct, feminine noun
Record 1, Abbreviations, French
Record 1, Synonyms, French
- centrifugation à l'équilibre en gradient de CsCl 2, record 1, French, centrifugation%20%C3%A0%20l%27%C3%A9quilibre%20en%20gradient%20de%20CsCl
correct, feminine noun
- centrifugation en CsCl 2, record 1, French, centrifugation%20en%20CsCl
correct, feminine noun
Record 1, Textual support, French
Record number: 1, Textual support number: 1 OBS
Il est possible d'identifier l'ADN plasmidique et de le séparer de l'ADN chromosomique par centrifugation isopycnique en gradient de chlorure de césium. L'ADN plasmidique surenroulé est capable d'intercaler entre ses bases moins de bromure d'éthidium que l'ADN chromosomique linéaire. Cette fixation différentielle crée une différence de densité aboutissant au cours de la centrifugation à la formation dans le gradient de deux bandes séparées correspondant l'une à l'ADN chromosomique, l'autre à l'ADN plasmidique. L'ADN plasmidique ainsi purifié peut être conservé et analysé plus avant par microscopie électronique. 2, record 1, French, - centrifugation%20isopycnique%20en%20gradient%20de%20chlorure%20de%20c%C3%A9sium
Record 1, Spanish
Record 1, Textual support, Spanish
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