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AFFINITE ENZYME SUBSTRAT [1 record]
Record 1 - internal organization data 2011-01-10
Record 1, English
Record 1, Subject field(s)
- Biological Sciences
Record 1, Main entry term, English
- enzyme-substrate affinity
1, record 1, English, enzyme%2Dsubstrate%20affinity
correct
Record 1, Abbreviations, English
Record 1, Synonyms, English
Record 1, Textual support, English
Record number: 1, Textual support number: 1 CONT
A fine-structure analysis of the tyrosyl-tRNA synthetase from Bacillus stereothermophilus is revealing the subtle roles of hydrogen bonding in catalysis and specificity. Modification of the residues that hydrogen-bond with ATP and tyrosine shows how the energetics must be analysed in terms of an exchange reaction with solvent water. Based on this idea, and structural data, an enzyme of vastly improved enzyme-substrate affinity has been engineered. 2, record 1, English, - enzyme%2Dsubstrate%20affinity
Record 1, French
Record 1, Domaine(s)
- Sciences biologiques
Record 1, Main entry term, French
- affinité d'un enzyme pour un substrat
1, record 1, French, affinit%C3%A9%20d%27un%20enzyme%20pour%20un%20substrat
correct, feminine noun
Record 1, Abbreviations, French
Record 1, Synonyms, French
Record 1, Textual support, French
Record number: 1, Textual support number: 1 CONT
L'affinité d'un enzyme pour un substrat est égale à 1/Km. Si Km est élevé (il faut donc une concentration élevée en substrat pour avoir une vitesse égale à Vmax/2) l'affinité est faible; si Km est faible, l'affinité est grande (il suffit d'une faible concentration en substrat pour avoir une vitesse égale à Vmax/2). 1, record 1, French, - affinit%C3%A9%20d%27un%20enzyme%20pour%20un%20substrat
Record number: 1, Textual support number: 1 OBS
Lorsqu'un activateur allostérique se fixe au site allostérique, il s'ensuit une légère modification de la conformation de l'enzyme -- appelée transition allostérique -- (d'ailleurs réversible), qui entraîne une modification de la conformation au niveau du site actif (catalytique). Ce site acquiert une conformation plus propice à la fixation du substrat, il y a augmentation de l'affinité de l'enzyme pour le substrat (Km' <Km). 1, record 1, French, - affinit%C3%A9%20d%27un%20enzyme%20pour%20un%20substrat
Record 1, Spanish
Record 1, Textual support, Spanish
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