TERMIUM Plus®

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MARQUEUR POIDS MOLECULAIRE [1 record]

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Record 1 2006-07-07

English

Subject field(s)
  • Bioengineering
DEF

A solution of DNA molecules of different lengths used in agarose gel electrophoresis as a reference to estimate the size of unknown DNA [fragments].

OBS

Different DNA ladders are commercially available depending on expected DNA length. The 1kbp [kilo base pairs] ladder with fragments ranging from about 0.5 kbp to 10 or 12 kbp and the 100 bp [base pairs] ladder with fragments ranging from 100 bp to just above 1000 bp are the most frequent. DNA ladders are often produced by a suitable restriction digest of a plasmid. There are special DNA ladders for supercoiled DNA and RNA.

French

Domaine(s)
  • Technique biologique
CONT

La taille des fragments d'ADN est estimée par comparaison avec un marqueur de taille connue (selon les expériences, on a utilisé le «100bp DNA ladder» ou le «lambda-HindIII ladder» de Promega).

Spanish

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